The insights of metabolic regulations from this study will help to point out the factors that may change overall seed composition during seed development, and guide enhancement of protein and oil. Cadmium cd concentrations in durum wheat triticum turgidum l. Joint analysis of nearisogenic and recombinant inbred. Nonetheless, depending on the available resources and the purpose of the lines to be generated, several choices can be made to guide the design of such inbred populations. Transcriptomic analysis of wheat nearisogenic lines. The four modern upland cotton genotypescultivars were selected in 1995, and were. While the approach and methods seem to be appropriate the language, especially in. This is a new line that differs from its parent in only one genomic location.
Mar 12, 2015 near isogenic lines nils were developed to confirm and finemap three of the 16 qtl, and to develop hypotheses regarding mechanisms of resistance. Extensive genomic characterization of a set of nearisogenic. Recombinant inbred linesrils versus near isogenic lines nils. Near isogenic lines nils a good way of validating a putative qtl is by creating a near isogenic line nil. Antixenosis feeding preference bioassays were conducted in a greenhouse at the university of georgia all et al. Identification of nuclear genes controlling chlorophyll.
The nearisogenic lines for virescent yellow and wildtype green leaf sets were developed by a backcross breeding method using four modern upland cotton varieties line development was detailed by others 35. Molecular response to the pathogen phytophthora sojae. Jan 29, 20 an introgression program based on residual heterozygous lines allowed us to develop five pairs of maize zea mays l. Pm2b, nearisogenic line, blumeria graminis, markerassisted selection, wheat. Various cold tolerant near isogenic lines nils selected at the booting stage through backcrossing of a japonica landrace lijing2 cold tolerant with cold sensitive towada a. Isogenic definition is characterized by essentially identical genes.
Development of nearisogenic lines with different alleles. Genotypic and phenotypic characterization of a large, diverse. Construction and evaluation of nearisogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu lifeng, zhang hongliang, mu ping, qu yanying, li zichao. Home browse subjects architecture and architectonics biology and life sciences chemistry and chemical engineering materials and applied sciences medical and health sciences social sciences and law. They can be used to model any disease with a genetic foundation. The fact of similar genetic background and differences at the locus of a target gene between nils would facilitate to analyze the response to pathogen in. The glyphosate resistance of nearisogenic lines h7242 rr and benning rrlxy123 was verified by applying roundup herbicide at the label rate to 12 greenhouse plants. One important aspect for understanding drought tolerance is the response of root growth and development to waterdeficit conditions. These advanced backcross lines are considered pre near isogenic lines because they are sister lines derived from a single family segregating for drought tolerance.
The dendrogram showed two distinct clusters at similarity coefficient of 33 % among the 24 rice entries. Structure of starches extracted from near isogenic wheat lines. In addition, a pair of nearisogenic lines nilsa clubrootresistant cr bjn32 line and a clubrootsusceptible bjn32 linewere developed for b. B73mo17 nearisogenic lines demonstrate dispersed structural. Such populations consist of lines containing a single fragment or a small number of genomic introgression fragments from a donor parent into an otherwise homogeneous genetic. We thus sequenced, analyzed, and compared the transcriptomes of 10 near isogenic lines nils, each with a unique rps geneallele, and the susceptible parent williams, pre. The near isogenic deletion lines were developed from mutant lines of the wheat cultivar olympic, null at the glub1 locus, and an isogenic line of the cultivar gabo, null at the glua1 and glud1 loci. To this effect, two gm maize varieties gm bt, gm rr, each containing a single insert, were subjected to comparative profiling, using the near. Materials and methods near isogenic lines were obtained by backcrossing the susceptible bread wheat cultivar. A set from this series of deletion lines was obtained from dr. In this way, the effects of the qtl can be isolated from other genetic effects.
To understand the physiological processes responsible for elevated cd accumulation in shoots and grain, cd uptake and translocation were studied in seedlings of a pair of nearisogenic. Molecular response to the pathogen phytophthora sojae among. Cereals are among the most important group of cultivated plants for food production worldwide. Agronomy free fulltext nearisogenic lines of japonica.
Here we describe the results of the detailed genomic characterization of these nils that we undertook to. A novel arraybased genotyping platform was used to score genotypes of over 7,000 loci in 100 nils with b73 as the recurrent parent and 50 nils with mo17 as the recurrent parent. As an intermediate step in the preparation of nearisogenic lines representing individual qtl alleles and combinations of qtl alleles in a homogeneous genetic background, we developed a set of qtl introgression lines. Genetic engineering of agricultural crops has played an important role in crop improvement where it has been used. This thesis provides insights to molecular mechanisms and regulatory networks as related to changes in seed protein content in nearisogenic soybean lines. Rice panicle plasticity in near isogenic lines carrying a qtl. Various cold tolerant nearisogenic lines nils selected at the booting stage through backcrossing of a japonica landrace lijing2 cold tolerant with cold sensitive.
Nearisogenic lines of triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during diuraphis noxia feeding, biology open, 2014, pp. Rice panicle plasticity in near isogenic lines carrying a. A set of nearisogenic lines of indicatype rice variety. This study addressed the response of rice panicle size and structure to limited light availability at plant level for nearisogenic lines nils with ir64 or irri146 backgrounds, carrying the qtl qtsn4 syn. Development of high tryptophan maize near isogenic lines. Use of nearisogenic wheat lines to determine the glutenin. An introgression program based on residual heterozygous lines allowed us to develop five pairs of maize zea mays l.
Zeigler2, surapong sarkarung2,2, itsuro takamurel and toshiro kinoshita 1 plant breeding institute, faculty of agriculture, hokkaido university, sapporo 060, japan. What is difference between isogenic and near isogenic line. They are provided with a genetically matched normal cell to provide an isogenic system to research disease biology and novel therapeutic agents. Jan 10, 2014 in this study, the transcriptomes of two near isogenic lines nils, one resistant and one susceptible to bacterial leaf pustule, were analyzed 0, 6, and 12 hpi and a total of 2,761 differentially expressed genes degs, including a set of defense response genes, were identified.
After two backcross and three selfing generations, six near isogenic lines nils with 93% recovery of the recurrent parent genome were created from one cross. These intermediate barley near isogenic ibison lines represent disease resistance qtl combined in one, two, and threeway. This is achieved when selection is made for only one locus of interest after each repeated backcross with a recurrent. Five f 7 rils from the mapping population were chosen as founders of hifs on the basis of being heterozygote for the 4a1 qtl region fig. However, little is known regarding the rpsinduced molecular responses to p. Cadmium uptake and translocation in seedlings of near. Near isogenic line are especially good for mendelising quantitative trait. View the article pdf and any associated supplements and figures for a period of 48 hours. B73mo17 nearisogenic lines demonstrate dispersed structural variation in maize. This thesis provides insights to molecular mechanisms and regulatory networks as related to changes in seed protein content in near isogenic soybean lines. Construction and evaluation of nearisogenic lines for major.
The nearisogenic lines nils inbred lines are usually developed for qtl mapping purpose. Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Results phenotypic characterization of the near isogenic lines and pleotropic effect of alm the i. Evaluation of pest vulnerability of benning soybean. The report nearisogenic lines nils of japonica rice revealed 2 new qtls for coldtolerance at booting stage describes the generation and analysis of near isogenic lines of the landrace linjing2 coldtolerant in the coldsensitive japonica background towada. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Fusarium head blight fhb is a disease caused predominantly by the fungal pathogen fusarium graminearum that affects wheat and other small. Microarray analysis of iron deficiency chlorosis in near isogenic soybean lines jamie a orourke 2 dirk v charlson 1 delkin o gonzalez 0 lila o vodkin 0 michelle a graham 4 5 silvia r cianzio 4 michael a grusak 3 randy c shoemaker 4 5 0 department of crop sciences, university of illinois urbanachampaign, urbana, illinois 61801 usa 1. Genome wide association studies can identify quantitative trait loci qtl putatively underlying traits of interest, and nested association mapping can further assess allelic series. Apr 14, 2004 cadmium cd concentrations in durum wheat triticum turgidum l. Genome wide association studies can identify quantitative trait loci qtl putatively underlying traits of interest, and nested association mapping can. Near isogenic lines of triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during diuraphis noxia feeding, biology open, 2014, pp. Construction and evaluation of near isogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu lifeng, zhang hongliang, mu ping, qu yanying, li zichao.
Rice blast is one of the most destructive diseases of rice. Apr 01, 20 the glyphosate resistance of nearisogenic lines h7242 rr and benning rrlxy123 was verified by applying roundup herbicide at the label rate to 12 greenhouse plants. Transcriptome analysis of brassica rapa nearisogenic lines. Nearisogenic lines nils a good way of validating a putative qtl is by creating a nearisogenic line nil. The near isogenic lines for virescent yellow and wildtype green leaf sets. Bwalm nearisogenic line is characterized by albino lemma base and the central part, however the upper part of the lemma and awns are green. Examining the transcriptional response in wheat fhb1 near. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference. These intermediate barley nearisogenic ibison lines represent disease resistance qtl combined in one, two, and threeway.
The most effective way of managing this disease is to develop resistant cultivars by introducing resistance genes into elite rice recipients. Genotypic and phenotypic characterization of a large. Differentiation of pathogenic races of rice blast fungus by using near isogenic lines with indica genetic backgroundl tsuyoshi inukai\ rebecca j. Microarray analysis of iron deficiency chlorosis in near. Nearisogenic lines of triticum aestivum with distinct modes. Please login to access your subscription or purchase a pay per view session. Nearisogenic lines of triticum aestivum with distinct. Proteomic and transcriptomic analysis of nearisogenic. Cottonseed protein, oil, and mineral nutrition in near. Here, transcriptome analysis of immature spike tissues in three lines nearly isogenic for q revealed.
Near isogenic lines nils, pairs of lines that are identical except for a single genomic segment, are ideal for this purpose szalma et al. Bwalm near isogenic line is characterized by albino lemma base and the central part, however the upper part of the lemma and awns are green. Finlay macritchie kansas state university, manhattan, kansas. Generation of multiple nearisogenic lines and rnaseq analysis we generated multiple nils segregating for the 4a1 qtl following a method based on hifs analysis 8. These two parents were used to produce a set of near isogenic lines nils with small regions of introgression into both backgrounds. Identification of molecular markers linked to leaf rust. The nils included 14 resistance genespish, pib, piz5, pizt, pi5t, piks, pik, pikh, pikm, pikp, pi1, pi7t, pita, and. These are also referred to as nearisogenic lines nils m onforte and t anksley 2000 or backcross inbred lines bils j euken and l indhout 2004. Near isogenic lines mtu 2547a781911 and mtu 2547a77111 showed 100 % similarity. Graduate thesis or dissertation quantitative resistance to. Production of nearisogenic lines and marked monosomic lines.
Recombinant inbred linesrils versus near isogenic lines. The insights of metabolic regulations from this study will help to point out the factors that may change overall seed composition during seed development, and guide enhancement of protein and oil accumulation in soybean. Twentyseven nearisogenic lines nils with the genetic background of a blastsusceptible variety, co 39, were developed by repeated backcrossing as a first set of a large number of differential varieties dvs with indicatype genetic background. Xu h, cao y, xu y, ma p, ma f, song l, li l and an d 2017 markerassisted development and evaluation of nearisogenic lines for broadspectrum powdery mildew resistance gene pm2b introgressed into different genetic backgrounds of wheat. Most r genes to blb have been introgressed into the background of the susceptible indica cultivar to develop a set of near isogenic lines nils, and some have been pyramided, either through classical breeding and markerassisted selection or through genetic engineering, to develop new plant types and nils sanchez et al. Bacterial and fungal leaf microbiomes of the resulting near. The dendrogram showed two distinct clusters at similarity coefficient of 33 %. Low temperature stress severely hampers rice productivity, and hence elaborating chillingmediated physiochemical alterations and unravelling cold tolerance pathways will facilitate cold resilient rice breeding. Three genes from alien species showed segregation distortion.
Use of nearisogenic wheat lines to determine the glutenin composition and functionality requirements for flour tortillas suchismita mondal, michael tilley, juma novi alviola, ralph d. As an intermediate step in the preparation of near isogenic lines representing individual qtl alleles and combinations of qtl alleles in a homogeneous genetic background, we developed a set of qtl introgression lines. Sixteen nearisogenic lines nils carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat triticum aestivum cv. Peiffer, gregory allan, utilization of nearisogenic lines to identify genes underlying ironefficiency qtl 2011. Production of nearisogenic lines and marked monosomic. Pdf the development and characterization of nearisogenic. To develop the nil mapping population, cross of a donor. Using near isogenic barley lines to validate deoxynivalenol. Phytophthora root and stem rot prr of soybean, caused by phytophthora sojae, is controlled by rps genes.
The results pertaining to these studies are presented here. Jan 10, 2014 phytophthora root and stem rot prr of soybean, caused by phytophthora sojae, is controlled by rps genes. These varieties were compared in a single location, petit, during three consecutive years. A set of lines that are genetically identical, except for one or a few loci. Markerassisted development and evaluation of nearisogenic. To understand the physiological processes responsible for elevated cd accumulation in shoots and grain, cd uptake and translocation were studied in seedlings of a pair of near isogenic durum wheat lines, high and low for cd. Does phloembased resistance to aphid feeding affect host. Results phenotypic characterization of the nearisogenic lines and pleotropic effect of alm the i. Sixteen near isogenic lines nils carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat triticum aestivum cv. Using the marker identified for that qtl, backcrosses are made to the recurrent parent usually the cultivated variety, in a wide cross.
Using near isogenic barley lines to validate deoxynivalenol don qtl previously identified through association analysis navara, stephanie lynn 20 view download file. Although near isogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant nils in the western flower thrips, frankliniella occidentalis pergande thysanoptera. This study addressed the response of rice panicle size and structure to limited light availability at plant level for near isogenic lines nils with ir64 or irri146 backgrounds, carrying the qtl qtsn4 syn.
Extensive genomic characterization of a set of near. Zeigler2, surapong sarkarung2,2, itsuro takamurel and toshiro kinoshita 1 plant breeding institute. Generation of multiple near isogenic lines and rnaseq analysis we generated multiple nils segregating for the 4a1 qtl following a method based on hifs analysis 8. The development of near isogenic lines nils through repeated backcrossing of genetically distinct parental lines is rather straightforward. Isogenic article about isogenic by the free dictionary. The near isogenic lines nils inbred lines are usually developed for qtl mapping purpose. Near isogenic lines can be derived through repeated backcrossing to a recurrent parent. Pm2b, near isogenic line, blumeria graminis, markerassisted selection, wheat.
Utilization of nearisogenic lines to identify genes. While the approach and methods seem to be appropriate the language. Isogenic lines to fusarium graminearum infection and deoxynivalenol treatment anna n. Nearisogenic lines for maize disease resistance qtl. To allow detailed study of quantitative trait loci qtl, it is necessary to create near isogenic lines nils that differ only for the qtl of interest. Nearisogenic lines of japonica rice revealed new qtls for. Such lines can be created by crossing a donor line containing a gene or trait of interest with a recurrent parent to produce a heterozygous f1, and then repeatedly backcrossing the offspring to the recurrent parent bc1, bc2, etc, retaining the donor gene or trait in each successive generation. These two parents were used to produce a set of nearisogenic lines nils with small regions of introgression into both backgrounds. We thus sequenced, analyzed, and compared the transcriptomes of 10 near isogenic lines nils, each with a unique rps geneallele, and the. This is a new line that differs from its parent in. Graduate thesis or dissertation quantitative resistance. Isogenic human disease models are a family of cells that are selected or engineered to accurately model the genetics of a specific patient population, in vitro. Microarray analysis of iron deficiency chlorosis in nearisogenic soybean lines jamie a orourke 2 dirk v charlson 1 delkin o gonzalez 0 lila o vodkin 0 michelle a graham 4 5 silvia r cianzio 4 michael a grusak 3 randy c shoemaker 4 5 0 department of crop sciences, university of illinois urbanachampaign, urbana, illinois 61801 usa 1. Evaluation of pest vulnerability of benning soybean value.
Field management and growth conditions a field experiment was conducted in 2014 and repeated in 2015 in stoneville, ms. In this study, the nearisogenic lines nils of six resistance alleles of the piz locus pizt, pi2, pigm, pi40, pi9 and piz were constructed with yangdao 6 as genetic. Trichothecene mycotoxins, such as deoxynivalenol don, accumulate during infection and increase pathogen virulence and decrease grain quality. The near isogenic lines for virescent yellow and wildtype green leaf sets were developed by a backcross breeding method using four modern upland cotton varieties line development was detailed by others 35.
Use of nearisogenic lines derived by backcrossing or selfing to map qualitative traits. Status message this article requires a subscription. Construction and evaluation of nearisogenic lines for. The development and characterization of nearisogenic and pyramided lines carrying resistance genes to brown planthopper with the genetic background of japonica rice oryza sativa l. Xu h, cao y, xu y, ma p, ma f, song l, li l and an d 2017 markerassisted development and evaluation of near isogenic lines for broadspectrum powdery mildew resistance gene pm2b introgressed into different genetic backgrounds of wheat. The present study, using near isogenic lines, was conducted to provide an alternative estimate of yield reduction caused by the hessian fly in morocco. Full light and shading in the greenhouse and two population densities in the field were implemented.
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